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Antibodies bound to the blocking peptide not bind for the epitope within the target protein. By comparing the staining from the blocked antibody compared to the antibody alone, you can see which staining is distinct.
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This guidebook outlines further more attainable optimization of mobile and immunoassay parameters to make certain the very best final results are...
This guideline outlines more doable optimization of mobile and immunoassay parameters to make certain the very best results are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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Blocking peptides are peptides that bind specifically to the focus more info on antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which add on the temporal coordination of each mitotic event. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates at the G1-S period boundary and is also degraded as cells development by way of S phase.
Overexpression of this gene has been noticed in many tumors, which results in chromosome instability, and thus may well lead to tumorigenesis. This protein was observed to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a critical role in promoting mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been explained. Two further splice variants were described but comprehensive nucleotide sequence facts is not but out there.
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